Research Studies

- Male and female - Overweight and obese BMI of 25-40 kg/m2and weight stable over the previous 6 months. - Age, 18-45 years old. - Fasting glucose of 100-125 mg/dL or fasting HbA1c of 5.7-6.4%, or 2h OGTT blood glucose of 140-199mg/dL based on the American Diabetes Association criteria for pre-diabetes. -Less than 150 minutes of moderate-to-vigorous physical activity (MVPA) per week and more than 6 hours of sitting time per day, as self-reported by the volunteers using the IPAQ.

Inclusion Criteria: -Male and female - Overweight and obese BMI of 25-40 kg/m2and weight stable over the previous 6 months. - Age, 18-45 years old. - Fasting glucose of 100-125 mg/dL or fasting HbA1c of 5.7-6.4%, or 2h OGTT blood glucose of 140-199mg/dL based on the American Diabetes Association criteria for pre-diabetes. - Less than 150 minutes of moderate-to-vigorous physical activity (MVPA) per week and more than 6 hours of sitting time per day, as self-reported by the volunteers using the IPAQ. - Less than 6500 of steps per day as measured by a pedometer over 5 days (at least 1 weekend day). - Passing medical and physical screening, and analysis of blood and urine screening samples. - Low-moderate caffeine use (<3 cups/day). - Agree to refrain from alcohol and caffeine for 72 h before and during the inpatient CTRC visits. - Agree to refrain from any other structured exercise than the physical activity prescribed in each arm of the study. - Agree to eat control diets for 3 days before and during the CTRC visits; - Agree to refrain from taking any over-the-counter (including nonsteroidal anti-inflammatory drugs) or prescribed medication (apart from oral contraceptives) for 3 days prior to the inpatient CTRC visits; - Agree to wear a Fitbit activity monitor and upload data on the website on a daily basis for the whole duration of the study. - Agree to follow the physical activity interventions and to be randomly assigned to one of the two arms of the study. - Agree to complete all the study procedures. Exclusion Criteria: - Pregnancy, breast-feeding or post-menopause for women. - Being considered unsafe to participate as determined by the study physician (Dr. Bessesen). - Ever having a history of systemic, psychiatric, neurological disease, or drug and alcohol abuse. - History of cardiovascular disease, diabetes, uncontrolled hypertension, untreated thyroid, renal, hepatic diseases, dyslipidemia or any other medical condition affecting weight or lipid metabolism. - Being positive for human immunodeficiency virus or hepatitis B or C. - Taking medications affecting weight, triglycerides, energy intake/energy expenditure, or sleep in the last 3 months. - Having abnormal blood chemistry and/or hematology as deemed significant by the study physician. - Having a positive drugs of abuse urine screen at screening or upon entry into the laboratory session. - Being a smoker or having been a smoker in the 3 months prior to their screening visit. - Having donated over 400 mL of blood within 3 months (90 days) of screening for the study; - Working night shifts or traveling across more than 2 time zones within 1 month of and throughout the study. - Not completing the trial days of BREAK and ONE during the screening period to assess the willingness and ability of the participant to perform each of the interventions.